Innovations In Clinical Neuroscience

JAN-FEB 2017

A peer-reviewed, evidence-based journal for clinicians in the field of neuroscience

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Innovations in CLINICAL NEUROSCIENCE [ V O L U M E 1 4 , N U M B E R 1 – 2 , J A N U A R Y – F E B R U A R Y 2 0 1 7 ] 42 anxiolytic effects in various animal m odels, and increase incentive motivational responses in humans and rodents. 42 In this study, we investigated the behaviorial effects via the CA1 h ippocampal region of an IF diet versus an ad libitum (AL) diet on albino mice who were acutely administered a neurotoxic dose of MDMA. Since MDMA is commonly used as a recreational drug, especially amound young populations, this study provides insight into the potential use of CR diet as a tool for drug addiction treatment. Additional research is warranted. METHODS Animals. All the experiments were carried out on male albino mice (N=140). The animals were purchased from Tehran Pasteur Institute. They were housed in groups of eight, and all were maintained under constant temperatures of 22 to 24 ºC and a 12 hours of light/12 hours of dark cycle (7am–7pm or 0700– 1900 hours). The animals were maintained under these conditions for about one week before the starting the experiments. Animal treatment and experiments were carried out according to the policies of the Institutional Animal Care and Use Committee for the Society for Neuroscience (Washington, DC). The research project was approved by the Medical Research Ethics Committee of the Shiraz University of Medical Sciences in Tehran, Iran. Drug. MDMA tablets were provided by the Antinarcotics Police (Applied Research Unit, Tehran, Iran). Purity of the compound, (±)-MDMA hydrochloride, (DL -3,4- methylenedioxymethamphetamine) was verified by LC-mass (quantitative analysis) and GC-mass (qualitative analysis) by the same organization as per our request, and no contaminants were detected (see Khajeamiri et al 42 ). (±)-MDMA hydrochloride was dissolved in 0.9 percent NaCl to the concentrations of 20 and 60mg/kg. These doses were chosen according to the reports published previously. 22,44 Since small mammals eliminate drugs at a faster rate than large mammals, to achieve a similar effect seen in humans, FIGURE 2. Time percentage spent in open arms (in seconds) of plus-maze of AL and IF groups, 30 minutes after MDMA gavage. Ad libitum (AL), Intermittent feeding (IF), Control (C).(Analysis was done by one-way ANOVA; significance was determined by post hoc Tukey test) *Significant difference between the IF and AL groups (20 mg/kg).(*** p < 0.001) #Significant difference between the IF and AL groups (60 mg/kg).(### p < 0.001) ★Significant difference between the IF C and IF groups (20 and 60 mg/kg).(p < 0.001) ★★Significant difference between the AL C and AL groups (20 and 60 mg/kg).(p< 0.001) FIGURE 3. Time percentage spent in open arms (in seconds) of plus-maze AL-AL, AL-IF, IF- IF and IF-AL subgroups, 12 days after MDMA gavage. Ad libitum (AL), Intermittent feeding (IF), Control (C).(Analysis was done by pairwise comparison one-way ANOVA followed by post hoc Tukey test). *Significant difference between the IF-IF and IF-AL subgroups (20 mg/kg).(*** p < 0.001) ^Significant difference between the AL-AL and AL-IF subgroups (20 mg/kg).(^^ p< 0.01) #Significant difference between the AL-AL and AL-IF subgroups (60 mg/kg).(### p < 0.001) +Significant difference between the IF-IF and IF-AL subgroups (60 mg/kg).(+++ p<0.001)

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